Rnaseq qc
A package manager for your agent skills — one canonical library, loaded on demand, never all at once.
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Run quality control on bulk RNA-seq data — adapter trimming, FastQC, alignment-rate checks, and a per-sample QC summary table. Use when the user has raw FASTQ files and needs a QC pass before differential expression.
SKILL.md
0.6 KB, as published. Nobody here has run it
RNA-seq QC
Quality-control workflow for bulk RNA-seq before differential expression.
Steps
- Run
fastqcon every FASTQ file. - Trim adapters with
fastp. - Align and capture the alignment rate per sample.
- Emit a per-sample QC summary table; flag samples below 70% alignment.
See reference/thresholds.md for the exact cutoffs.